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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in many tumors, which ends up in chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant position in marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be described. Two further splice variants ended up documented but in depth nucleotide sequence facts is not really nonetheless out there.
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Blocking peptides are peptides that bind exclusively for the goal antibody and block antibody binding. These peptide generally consists of the epitope recognized via the antibody.
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This guide outlines even further achievable optimization of cellular and immunoassay parameters to ensure the absolute best success are...
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This guidebook outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
Antibodies sure to the blocking peptide no longer bind to the epitope to the concentrate on protein. By comparing the staining through the blocked antibody as opposed to the antibody by itself, one can see which staining is precise.
Target Details Cyclin E belongs towards the really conserved cyclin household, whose customers are characterised by a spectacular periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation designs which lead towards the temporal coordination of each mitotic occasion. Cyclin E types a fancy with and functions like a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is particularly degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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